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Microscopic Examination Unknown 52 was observed under oil immersion using the brightfield light microscope 1000x magnification after conducting Gram stain technique Escherichia coli (Gram negative rods) preprepared slide was the negative control while the Staphylococcus epidermidis (Gram positive cocci) preprepared slide provided theA Gram stain of mixed Staphylococcus aureus (S aureus ATCC , Grampositive cocci, in purple) and Escherichia coli (E coli ATCC , gramnegative bacilli, in red), the most common Gram stain reference bacteria Gram stain or Gram staining, also called Gram's method,Staphylococcus is a genus of Gram ve, round shape bacteria that are arranged in grapelike clusters The organisms of this genus are the commonest cause of suppurative lesions It was first isolated by Sir Alexander Ogston and he gave the name Staphylococcus for the typical arrangement of the cocci in grapelike clusters Identifying Bacteria 1 Bms 503 Unh Elizabeth Valcourt Bms 504 05 28 September 17